Author/Authors :
Wang، نويسنده , , Yu and Chu، نويسنده , , Wangsheng and Yang، نويسنده , , Feifei and Yu، نويسنده , , Meijuan and Zhao، نويسنده , , Haifeng and Gong، نويسنده , , Weimin and Dong، نويسنده , , Yuhui and Xie، نويسنده , , Yaning and Wu، نويسنده , , Ziyu، نويسنده ,
Abstract :
We used X-ray absorption near edge structure (XANES) spectroscopy to investigate the metal-dependent enzymatic activity of the peptide deformylase from Leptospira interrogans (LiPDF). Ab initio full multiple scattering calculations performed by MXAN are applied to obtain the local structure of the cobalt-containing LiPDF (Co-LiPDF) and zinc-containing LiPDF (Zn-LiPDF) around the metal sites in pH9.0 buffer solution. The result shows the cobalt-wat1 (the bond water molecule) distance of Co-LiPDF is 1.89 إ, much shorter than that of Zn-LiPDF, 2.50 إ. That is an essential factor for its low catalytic activity.
