Title of article
Genetic diversity of rhizobia from natural populations varies with the soil dilution sampled
Author/Authors
Bala، نويسنده , , Abdullahi and Murphy، نويسنده , , Phillip and Giller، نويسنده , , Ken E.، نويسنده ,
Pages
3
From page
841
To page
843
Abstract
Rhizobia were isolated from root nodules of three tree legumes inoculated with dilution series of two tropical soils. The diversity of the isolates obtained from different soil dilutions was assessed by PCR-RFLP analysis of the internally transcribed spacer (ITS) region between the 16S and 23S rRNA genes. In both soils there was a strong effect of dilution on the genetic diversity of the populations with apparent diversity decreasing with increasing dilution.
Keywords
Calliandra , Leucaena , Gliricidia , PCR-RFLP , most probable number , N2-fixation , Nodulation
Journal title
Astroparticle Physics
Record number
1993072
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