Author/Authors :
Stuhrmann، نويسنده , , H.B. and Burkhardt، نويسنده , , N. and Dietrich، نويسنده , , Anelm G. M. Jünemann، نويسنده , , R. and Meerwinck، نويسنده , , W. F. Schmitt، نويسنده , , M. and Wadzack، نويسنده , , J. and Willumeit، نويسنده , , R. and Zhao، نويسنده , , J. and Nierhaus، نويسنده , , K.H.، نويسنده ,
Abstract :
Polarized neutron scattering from polarized nuclear spin targets has been used to study the in situ structure of ribosomal components in solutions. The progress of this technique is substantiated by first results obtained from protonated tRNA and mRNA labels in a deuterated ribosome. The site of the tRNA could be determined uniquely, whereas there are several orientations of the [tRNA]2-mRNA complex which are compatible with the neutron scattering data. Similarly, a small, protonated mRNA fragment was studied. Its size favours a complicated path of the mRNA chain through the ribosome. The role of deuteron spin targets is discussed.
