Author/Authors :
Polikarpov، نويسنده , , I. and Oliva، نويسنده , , G. and Castellano، نويسنده , , E.E. and Garratt، نويسنده , , R.C. and Arruda، نويسنده , , P. and Leite، نويسنده , , A. and Craievich، نويسنده , , A.، نويسنده ,
Abstract :
The Brazilian National Synchrotron Light Laboratory - LNLS, will have a dedicated protein crystallography beamline. The beamline under construction includes cylindrical mirror and bent crystal monochromator focusing the high flux of synchrotron radiation in the horizontal plane at the position of the sample. The monochromatic radiation will be tuneable between 2.0 and 1.0 Å with the optimum wavelength at 1.3–1.6 Å, chosen with the aim of maximizing the photon flux from the bending magnets of the storage ring (1.37 GeV). Diffraction images will be recorded on a commercial image plate detector system with on-line readout. The beamline set-up will include cooler/chiller for the samples and biochemical lab for crystallization, heavy-metal soaks, crystal storage and mounting at 22°C and 4°C, will also be available. The facility, intended to serve the national and international community, is planned to be brought into operation in the second half of 1997. It is foreseen that the commissioning of the first protein crystallography beamline in Latin America will boost the number of protein structures determined locally and will increase the general interest of the molecular biology and biochemical research community of Brazil in this area.
