Title of article :
Ultrasound-mediated drug delivery using liposomes modified with a thermosensitive polymer
Author/Authors :
Ninomiya، نويسنده , , Kazuaki and Kawabata، نويسنده , , Shinya and Tashita، نويسنده , , Hiroyuki and Shimizu، نويسنده , , Nobuaki، نويسنده ,
Abstract :
Ultrasound-mediated drug delivery was established using liposomes that were modified with the thermosensitive polymer (TSP) poly(NIPMAM-co-NIPAM), which sensitized the liposomes to high temperatures. TSP-modified liposomes (TSP liposomes) released encapsulated calcein under 1 MHz ultrasound irradiation at 0.5 W/cm2 for 120 s as well as the case under incubation at 42 °C for 15 min. In addition, uptake of the drug released from TSP liposomes by cancer cells was enhanced by ultrasound irradiation. In a cell injury assay using doxorubicin (DOX)-loaded TSP liposomes and ultrasound irradiation, cell viability of HepG2 cells at 6 h after ultrasound irradiation (1 MHz, 0.5 W/cm2 for 30 s) with DOX-loaded TSP liposomes (TSP/lipid ratio = 1) was 60%, which was significantly lower than that of the control conditions such as DOX-loaded TSP liposomes alone and DOX-loaded intact liposomes under ultrasound irradiation.
Keywords :
Temperature-sensitive liposomes , DRUG DELIVERY , Ultrasound , Thermosensitive polymer , Cavitation
Journal title :
Astroparticle Physics