Author/Authors :
-، - نويسنده Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Jamshidzadeh, Akram , -، - نويسنده Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Gerash Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Gerash, Iran Heidari, Reza , -، - نويسنده Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Razmjou, Mojtaba , -، - نويسنده Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran Karimi, Forouzan , -، - نويسنده Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Moein, Mahmood Reza , -، - نويسنده Shiraz University of Medical Sciences, International Branch, Shiraz, Iran Farshad, Omid , -، - نويسنده Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Akbarizadeh, Amin Reza , -، - نويسنده Shiraz University of Medical Sciences, International Branch, Shiraz, Iran Houshangi Shayesteh, Mohammad Reza
Abstract :
Objective(s): Protective effects of different extracts and essential oil from Pimpinella anisum L. seeds were examined against carbon tetrachloride (CCl4)-induced toxicity. The parameters such as serum transaminases, lactate dehydrogenase activity, hepatic glutathione content, liver lipid peroxidation and histopathological changes of liver were assessed as toxicity markers. In the in vitro model of this study, markers such as cell viability, cellular reduced and oxidized glutathione and lipid peroxidation in HepG2 cells were evaluated.
Materials and Methods:Human liver cancer cell line HepG2 and male Sprague-Dawley rats were treated with extracts and essential oil, and markers of hepatotoxicity were investigated.
Results: The data revealed that the n-hexane extract, effectively attenuated CCl4‑induced toxicity in both in vitro and in vivo models in current investigation.
Conclusion: As the oxidative stress markers were ameliorated, it might be concluded that anise seed possesses protective effects probably due to its antioxidant constituents.
