Author/Authors :
Mahmoudi Rad، Mahnaz نويسنده Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mahmoudi Rad, Mahnaz , Mahmoudi Rad، Niki نويسنده Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mahmoudi Rad, Niki , Mirdamadi، Yasaman نويسنده Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mirdamadi, Yasaman
Abstract :
Background: The multifunctional transforming growth factor beta (TGF-B) is a glycoprotein that exists in three isoforms. TGF-B3 expression increases in fetal wound healing and reduces fibronectin and collagen I and III deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. Fibroblasts are key cells in the wound healing process. TGF-B3 plays a critical role in scar-free wound healing and fibroblast actions in the wound healing process. The aim of this study was to express the TGF-B3 gene (tgf-b3) in human foreskin fibroblasts (HFF’s).
Methods: We obtained HFF’s from a newborn and a primary fibroblast culture was prepared. The cells were transfected with TGF-B3-pCMV6-XL5 plasmid DNA by both lipofection and electroporation. Expression of TGF-B3 was measured by enzyme-linked immunosorbent assay (ELISA).
Results: The highest TGF-B3 expression (8.3-fold greater than control) was obtained by lipofection after 72 hours using 3 µl of transfection reagent. Expression was 1.4-fold greater than control by electroporation.
Conclusions: In this study, we successfully increased TGF-B3 expression in primary fibroblast cells. In the future, grafting these transfected fibroblasts onto wounds can help the healing process without scarring.
