Author/Authors :
Simrouni، M. M نويسنده Department of Aquatic Animal Health, College of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran , , Mesbah، M نويسنده Department of Aquatic Animal Health, College of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran , , Rajabi Memari، H نويسنده Center of Biotechnology Research, College of Agriculture, Shahid Chamran University, Ahvaz, Iran , , Seifi Abad Shapoori، M. R نويسنده Center of Virology Research, college of veterinary Medicine, University of Shahid Chamran University, Ahvaz, Iran , , PurMahdi Boroujeni، M نويسنده Department of Food Hygein, College of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran ,
Abstract :
White spot syndrome virus (WSSV) is a pathogen that causes high mortality in shrimp culture in the whole world. Sequence analysis of WSSV has shown similarity of WSSV isolates in different countries with exception of a few variable genomic loci. This study investigated the sequence variation of some Iranian WSSV isolates and previously identified isolates. Samples were collected during target surveillance and were feed, broodstock, post-larvae, artemia, crabs, and wild and cultured shrimp of northern Persian Gulf (Boushehr and Khuzestan provinces). The open reading frame (ORF) 94 sequence of different Iranian WSSV isolates were amplified using specific primers from positive samples. The ORFs 94 sequence of positive samples were sequenced and registered in the Gene Bank and then compared to other WSSV isolates. The number of repeat units in ORF94 showed that WSSV isolates were varied in number. There are SNPs (G and T) in position 48 of RUs that varies in different Boushehr and Khuzestan isolates. Also these sequences were compared to Gene Bank WSSV isolates and showed a high similarity ( > 90%) to Southeast Asian countries. To our knowledge this is the first report of sequence analysis in Iranian WSSV isolatesapplications.
