Author/Authors :
Beetz، نويسنده , , T. and Howells، نويسنده , , M.R. and Jacobsen، نويسنده , , C. and Kao، نويسنده , , C.-C. and Kirz، نويسنده , , J. and Lima، نويسنده , , E. and Mentes، نويسنده , , T.O. and Miao، نويسنده , , H. and Sanchez-Hanke، نويسنده , , C. and Sayre، نويسنده , , D. and Shapiro، نويسنده , , D.، نويسنده ,
Abstract :
An apparatus for diffraction microscopy of biological and materials science specimens is described. In this system, a coherent soft X-ray beam is selected with a pinhole, and the illuminated specimen is followed by an adjustable beamstop and CCD camera to record diffraction data from non-crystalline specimens. In addition, a Fresnel zone plate can be inserted to allow for direct imaging. The system makes use of a cryogenic specimen holder with cryotransfer capabilities to allow frozen hydrated specimens to be loaded. The specimen can be tilted over a range of ± 80 ∘ for three-dimensional imaging; this is done by computer-controlled motors, enabling automated alignment of the specimen through a tilt series. The system is now in use for experiments in soft X-ray diffraction microscopy.
Keywords :
X-ray microscopy , x-ray imaging , X-ray tomography , Diffraction microscopy , lensless imaging
