Effect of Various Levels of Cytokinin and Auxin for In-Vitro Regeneration of Banana Cultivars

An experiment was carried out to find out the effect of various levels of cytokinin and auxin for rooting of regeneration of banana through in vitro culture. Excised shoot tip of banana cv. Grande Naine and Jahaji were cultured on MS medium supplemented with cytokinin (BAP) and auxins (NAA and IBA). 4.5 mg/l was found as the best concentration of BAP in induction of numerous buds, among the various concentration tested (0-6.5 mg/l BAP). The treatment resulted in the highest number of buds (an average of 7.05 and 7.2) with highest mean length of 0.65 cm and 0.7 cm of shoots were recorded in 4.5 mg/l BAP concentration in Jahaji and in Grande Naine respectively. Shoot elongation resulted best in lower concentration of BAP (1.5 mg/l). For rooting, NAA and IBA were used individually in concentrations of 0, 0.1.0.2.1 mg/l. Cent percent rooting was achieved in medium with 1 mg/l NAA. This treatment also recorded highest number of functional roots (6.33 and 5.2) in Grande Naine and Jahaji respectively with moderate root length of (2-4)cm. it was recorded that ex-vitro survival of plantlets is 98% when they were subjected to secondary hardening in media mixture of pre sterilized topsoil: FYM: sand: vermicompost: cocopeat in ratio of 1:1:1/2:1/2:1 (V/V)filled in black polybags under 75% shade net condition