Author/Authors :
Huang، Dong Liang نويسنده Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences , , QIN، Cui-Xian نويسنده Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences , , CHEN، Zhong-Liang نويسنده Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences , , GUI، Yi-Yun نويسنده Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences , , Wang، Miao نويسنده , , Zhou، Jianhui نويسنده , , LIAO، Qing نويسنده Guangxi Academy of Agricultural Sciences, Nanning 530007, China , , Yang، Li-Tao نويسنده State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture , , Li، Yang-Rui نويسنده Guangxi Key Laboratory of Sugarcane Genetic Improvement; Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Guangxi ,
Abstract :
To date, it is difficult to transform sugarcane
using Agrobacterium because sugarcane belongs to
Monocotyledonae, which are not native host of
Agrobacterium. To establish a highly efficient sugarcane
transformation system, the optimal concentration of
antibiotic (Km) was screened for subculture and
differentiation of sugarcane calli, as well as seedling rooting,
respectively. The factors that influenced sugarcane
transformation, such as pre-culture time, infection time and
co-culture time were also studied. The results showed that the
optimal concentrations of antibiotic (Km) for subculture,
differentiation and seedling rooting were 300 mg/L, 40 mg/L
and 20 mg/L, respectively. The optimal pre-culture time,
infection time and co-culture time were 4 days, 30 min and 4
days, respectively. PCR detection showed that 61.6% of
resistant plants were transgenic. This work lays a solid
foundation for further study on gene function and transgenic
research of sugarcane.
