Inhibition of Pectinolytic and Cellulolytic Enzymes of Fusarium oxysporum by Trichoderma spp and Pseudomonas fluorescens on Arachishypogaea.L

The present study examined the effects of culture filtrates of Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescens on Fusarium oxysporum infecting Arachis hypogaea. Fusarium wilt diseases caused by the fungus Fusarium oxysporum lead to significant yield losses of crops. Experiments were conducted on the effect of culture filtrates of T.viride (1%), T.harzianum (1.5%), and P. fluorescens (2%) on Pectinolytic and Cellulytic enzymes produced by Fusariumoxysporum on Arachishypogaea. The activity of Poly Methyl Esterase (PME), endo polymethylgalacturonase (PMG), and exo polymethylgalacturonase (PMG), produced by Fusarium oxysporum in the leaves of infected Arachis hypogaea was higher, when compared to control plants. Maximum inhibition of above pectinolytic enzymes (PME, endo and, exo PMG) in the plants sprayed with T. viride was followed by T.harzianum-sprayed plants and P.fluorescens- sprayed plants.The cellulolytic enzyme activity 1,4B endo, -glucanase,1,4B exo-glucanase, and the cellobiase enzyme activity in the leaves of infected Arachis hypogaea was higher compared to that of control plants. Activity of cellulolytic enzymes(1,4B endo, -glucanase,1,4B exo-glucanase, cellobiase) was inhibited significantly by T.viride-sprayed plants followed by T.harzianum-sprayed plants and P.fluorescens- sprayed plants. Of all the treatments, T. viride treatment showed higher rate of inhibition of Pectinolytic and Cellulytic enzymes followed by that of T.harzianum and P.fluorescens. This present study indicates that culture filtrate of T.viride(1%) is the best biocontrol agent in the inhibition of Fusarium oxysporum causing Fusarium wilt of Arachis hypogaea .L