Author/Authors :
-، - نويسنده Department of Food Science, Faculty of Biosystems Engineering, University College of Agriculture, University of Tehran, P.O. Box 31585-4314, Karaj, I.R. Iran Safari, Mohammad , -، - نويسنده Department of Food Science and Agricultural Chemistry, McGill University 21,111 Lakeshore, Ste. Anne de Bellevue, Québec, Canada, H9X 3V9 Karboune, Salwa , -، - نويسنده Department of Food Science and Agricultural Chemistry, McGill University 21,111 Lakeshore, Ste. Anne de Bellevue, Québec, Canada, H9X 3V9 Kermasha, Selim
Abstract :
Lipase from Candida antarctica, fixed on macroporous acrylic resin, has been used for the intermediate production of mono- and diolein by hydrolysis of triolein. The effect of altering concentrations of triolein and glycerol and the function of the molecular sieve on the hydrolysis reaction of triolein were investigated. The highest hydrolysis yield was observed for the utmost concentration of triolein, which gave a hydrolysis optimum at the lower reaction time. Raising the concentration of triolein resulted in a 13.7 times increase in mono- and diolein during reaction period. Addition of glycerol to the reaction mixture had a considerably higher positive effect on the production of monolein than that of diolein. The use of a molecular sieve in the mixture was found to be the most effective environment tested, which demonstrated high activity and excellent selectivity toward the formation of intermediate monolein.
