Author/Authors :
Xinmin، نويسنده , , Wang and Ruili، نويسنده , , Chun Zhang and Zhihua Wang ، نويسنده , , Lv and Yuanhong، نويسنده , , Wang and Tingfu، نويسنده , , Jiang، نويسنده ,
Abstract :
A simple method for the determination of glucosamine and lactose in milk-based formulae by high-performance liquid chromatography (HPLC) with refractive index detector was developed and validated. Samples were cleaned up just by protein precipitation before HPLC analysis. Separation was achieved with a Shodex Asahipak NH2P-50 column. The method showed good linearity, sensitivity, precision and recovery, and proved very simple and rapid for routine analysis since separation was completely achieved at 10 min.
Keywords :
HPLC , Milk-based formulae , glucosamine , Lactose
