Author/Authors :
-، - نويسنده Department of Industrial Biotechnology, Dr. M.G.R. Educational and Research Institute, Dr. M.G.R. University,
Chennai-600 095, India Perumal, Sathiya Moorthi , -، - نويسنده Department of Industrial Biotechnology, Dr. M.G.R. Educational and Research Institute, Dr. M.G.R. University,
Chennai-600 095, India Munuswamy, Deecaraman , -، - نويسنده Center for Advanced Studies in Botany, University of Madras, Chennai-600 025, India Sellamuthu, Periyar Selvam , -، - نويسنده Center for Advanced Studies in Botany, University of Madras, Chennai-600 025, India Kandasamy, Murugesan , -، - نويسنده Center for Advanced Studies in Botany, University of Madras, Chennai-600 025, India Puthupalayam Thangavelu, Kalaichelvan
Abstract :
The rate and efficiency of decolorization of dyes likeBlue CA, Black B133, and Corazol Violet SR were tested to evaluate white rot fungal strains. Trametes hirsuta and Pleurotus florida showed the greatest extent ofdecolorization on nutrient salt media. Maximum decolorization of 200 mg/l of Blue 133 was obtained by 4days old incubated Pleurotus florida followed byTrametes hirsuta after 6 days. An attempt was made toimprove the decolorization activity of both organismswith different concentrations of glucose 1 and 2%(w/v). The decolorization activity may be due to thelaccase enzyme of white rot fungi. The production ofthis enzyme was estimated using solid state fermentation with rice bran as a substrate. It was found that P.florida exhibited 0.175 U/ml of laccase activity followed0.126U/ml by T. hirsute, respectively. Decolourizationwas found to be more effective with P. florida in thepresence of 2% (w/v) glucose. Crude extract containing the laccase enzyme was isolated and confirmed bySDS PAGE
