Author/Authors :
-، - نويسنده Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, P.O. Box 65355-141, Ahvaz, I.R. Iran Hushiarian, Roozbeh , -، - نويسنده Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, P.O. Box 65355-141, Ahvaz, I.R. Iran Roayaei, Mohammad , -، - نويسنده Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, P.O. Box 65355-141, Ahvaz, I.R. Iran Galehdari, Hamid , -، - نويسنده Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, P.O. Box 65355-145, Ahvaz, I.R. Iran Seyfiabad Shapouri, Masoud Reza
Abstract :
Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. This region of 1356 bp was inserted into a eukaryotic expression plasmid, pCDNA4, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Plasmid DNA was transfected into COS-7 cell line and transient expression of VP2 from the constructed plasmid was characterized by dot blotting with a polyclonal antibody to IBDV.
