Saliva substitute in combination with high-concentrated fluoride toothpaste: Effects on demineralised dentin in vitro

Objectives m of this in vitro study was to assess the effects of saliva substitutes (modified with respect to calcium, phosphates, and fluorides) in combination with a high-concentrated fluoride toothpaste on demineralised dentin. s and after demineralisation of bovine dentin specimens (subsurface lesions; 37 °C, pH 5.0, 5 d), one-quarter of each specimenʹs surface was covered with nail varnish (control of sound/demineralised tissue). Subsequently, specimens were exposed to original Saliva natura (saturation with respect to octacalciumphosphate [SOCP]: 0.03; SN 0), or to three lab-produced Saliva natura modifications (SOCP: 1, 2, and 3; SN 1–3) for 2 and 5 weeks (37 °C). An aqueous solution (SOCP: 2.5) served as positive control (PC). Two times daily (2 min each), Duraphat toothpaste (5000 ppm F−; Colgate)/saliva substitute slurry (ratio 1:3) was applied gently. Differences in mineral losses (ΔΔZ) and lesion depths (ΔLD) between values before and after exposure were microradiographically evaluated. s both treatment periods specimens immersed in SN 0 revealed significantly higher mineral losses (lower ΔΔZ values) and lesion depths (lower ΔLD) compared to PC (p < 0.05; ANOVA). After 5 weeks, specimens stored in SN 1 and 2 showed significantly higher mineral losses compared to PC (p < 0.05), while those stored in SN 3 showed similar results (p > 0.05). No differences in mineral loss could be observed between SN 2 and 3 (p > 0.05). sions the conditions of this limited protocol, the combination of Saliva natura solutions slightly saturated with respect to OCP in combination with a high-concentrated fluoride toothpaste enabled remineralisation of dentin in vitro.