Author/Authors :
Hegazi، Ghada A. نويسنده Department of Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt , , Zayed، Mona S. نويسنده Department of Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt , , Salem، Hanaa M. نويسنده Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Cairo, Egypt , , Ibrahim، Walaa M. نويسنده Tissue Culture Unit, Department of Plant Genetic Resources, Ecology and Dry Land Agriculture Division, Desert Research Center, 11753 El-matarya, 1 Mathaf El-matarya St., El-matarya, Cairo, Egypt ,
Abstract :
Jojoba or Hohoba (Simmondsia chinensis (Link) Schneider) is an economically important plant in arid
and semi-arid areas. Micropropagation is a highly recommended strategy for obtaining Jojoba elite
clones. The present investigation aimed to study the effect of explant type and sequential subcultures
on the in vitro multiple shoots formation of Jojoba. Four explants form female mature plants were used
for the in vitro establishment of Jojoba; shoot tips and terminal, sub-terminal and basal stem node
segments. It was found that Murashige and Skoog (MS) medium supplemented with 6-
benzylaminopurine (BA) at 1.25 mg/l could be a promising treatment for the in vitro establishment of
all explant types. On the other hand, BA at 2 mg/l is the most promising concentration for shoot
multiplication, which gave higher values of mean number of axillary shoots during all successive
subcultures than the other treatments. It was also found that the mean number of axillary shoots
increased till the 4th subculture and then it decreased in the 5th and 6th subcultures. By comparing the
different explant types of Jojoba, shoot tips recorded the highest mean number and length of axillary
shoots.
