Micropattern of antibodies imaged by shear force microscopy: Comparison between classical and jumping modes

Quartz tuning fork devices are increasingly being used as nanosensors in Scanning Probe Microscopy. They offer some benefits with respect to standard microfabricated cantilevers in certain experimental setups including the study of biomolecules under physiological conditions. In this work, we compare three different working modes for imaging micropatterned antibodies with quartz tuning fork sensors: apart from the classical amplitude and frequency modulation strategies, for first time the jumping mode is implemented using tuning forks. Our results show that the molecules suffer less degradation when working in the jumping mode, due to the reduction of the interaction forces.