Title of article :
Evaluation of charging on macromolecules in electron cryomicroscopy
Author/Authors :
Brink، نويسنده , , JACOB HOLSINGER SHERMAN، نويسنده , , Michael B and Berriman، نويسنده , , John H. Chiu، نويسنده , , Wah، نويسنده ,
Abstract :
We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam “footprints” on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.
Keywords :
Imaging charged samples , cryomicroscopy , Defocused diffraction , Secondary electron emission , Electrical breakdown , Specimen current
Journal title :
Astroparticle Physics
