Author/Authors :
Chen، Yan نويسنده Department of Obstetrics and Gynecology of the Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China , , Li، Dong نويسنده Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China , , Zhang، Zhe نويسنده , , Takushige، Natsuko نويسنده Department of Obstetrics and Gynaecology, University of Sydney, Sydney, NSW, 2006, Australia , , Kong، Bei-Hua نويسنده Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China , , Wang، Guo-Yun نويسنده Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China ,
Abstract :
Background: Endometriosis is a common, benign, oestrogen-dependent, chronic gynaecological
disorder associated with pelvic pain and infertility. Some researchers have
identified nerve fibers in endometriotic lesions in women with endometriosis. Mesenchymal
stem cells (MSCs) have attracted interest for their possible use for both cell and
gene therapies because of their capacity for self-renewal and multipotentiality of differentiation.
We investigated how human umbilical cord-MSCs (hUC-MSCs) could affect
nerve fibers density in endometriosis.
Materials and Methods: In this experimental study, hUC-MSCs were isolated from
fresh human umbilical cord, characterized by flow cytometry, and then transplanted into
surgically induced endometriosis in a rat model. Ectopic endometrial implants were collected
four weeks later. The specimens were sectioned and stained immunohistochemically
with antibodies against neurofilament (NF), nerve growth factor (NGF), NGF
receptor p75 (NGFRp75), tyrosine kinase receptor-A (Trk-A), calcitonin gene-related
peptide (CGRP) and substance P (SP) to compare the presence of different types of nerve
fibers between the treatment group with the transplantation of hUC-MSCs and the control
group without the transplantation of hUC-MSCs.
Results: There were significantly less nerve fibers stained with specific markers we
used in the treatment group than in the control group (p < 0.05).
Conclusion: MSC from human umbilical cord reduced nerve fiber density in the treatment
group with the transplantation of hUC-MSCs.
