Increase in γϖ T cells in the ruminal mucosa of reindeer calves (Rangifer tarandus tarandus L.) induced by baled grass silage

Leukocytes in the forestomach mucosa of reindeer (Rangifer tarandus tarandus L.) were investigated by immunoperoxidase staining of cryostat sections, using monoclonal antibodies against antigens on sheep leukocytes. Mucosal samples from three free-ranging reindeer calves were compared with samples from three calves fed baled grass silage previously shown to induce increased frequency of lesions in the ruminal epithelium. In both groups, MHC-II + cells and γϖ T cells were observed, located within or just below the basal layer of the stratified epithelium. Computer-assisted morphometric analysis showed that the number of γϖ T cells in the ruminal mucosa was higher in the silage-fed than in the free-ranging animals. No marked difference in number of MHC-II + Langerhans cells was observed between the groups.