Title of article
Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies
Author/Authors
Mateo، نويسنده , , A. and Perez de la Lastra، نويسنده , , J. and Moreno، نويسنده , , A. and Dusinsky، نويسنده , , R. and Bilka، نويسنده , , F. Xavier Simon، نويسنده , , M. and Horovska، نويسنده , , L. and Llanes، نويسنده , , D.، نويسنده ,
Pages
8
From page
363
To page
370
Abstract
A panel of 18 monoclonal antibodies (mAbs) defined by the third workshop as specific for platelets, clustered in three preliminary groups: PC7, PC13 and PC27. These mAbs were further analysed by immunoprecipitation using extracts of iodinated and biotinylated peripheral blood mononucleated cells (PBMC) and platelets. We could confirm the existence of mAbs with specificities to WC9 (in PC7) and CD41/61 (in PC13). Two mAbs formed a new cluster, WC13, which may be homologous to human CD31 (in PC27). The influence of EDTA and thrombin on the expression of the different antigens on the platelet membrane was assessed by flow cytometry (FCM) analysis, as well as cross-reactivity with platelets from different species.
Journal title
Astroparticle Physics
Record number
2053794
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