Author/Authors :
Masuda، نويسنده , , Kenichi and Tsujimoto، نويسنده , , Hajime and Fujiwara، نويسنده , , Shunsuke and Kurata، نويسنده , , Keigo and Hasegawa، نويسنده , , Atsuhiko and Taniguchi، نويسنده , , Yoshifumi and Yamashita، نويسنده , , Kohei and Yasueda، نويسنده , , Hiroshi and DeBoer، نويسنده , , Douglas J. and de Weck، نويسنده , , Alain L. and Sakaguchi، نويسنده , , Masahiro، نويسنده ,
Abstract :
The present study investigated IgE-reactivity to two major Japanese cedar (Cryptomeria japonica, C. japonica) pollen allergens (Cry j 1 and Cry j 2) in dogs with atopic dermatitis by use of a fluorometric ELISA. The serum samples from 27 dogs that showed IgE-sensitivity to crude C. japonica pollen allergen by ELISA were tested for specific IgE to the two major allergens. All 27 dogs had anti-Cry j 1 IgE, and 10 (37%) had anti-Cry j 2 IgE. Inhibition of binding of dog specific IgE to crude C. japonica pollen allergen was carried out by addition of Cry j 1. When serum samples containing anti-Cry j 1 IgE but no anti-Cry j 2 IgE were incubated with Cry j 1, specific IgE binding to crude C. japonica pollen allergen was almost abolished. These findings suggest that Cry j 1 is a major allergen in dogs.
Keywords :
atopic dermatitis , dog , IGE , Japanese cedar pollen
