Title of article
A 7872 cDNA microarray and its use in bovine functional genomics
Author/Authors
Everts، نويسنده , , Robin E. and Band، نويسنده , , Mark R. and Liu، نويسنده , , Z. Lewis and Kumar، نويسنده , , Charu G. and Liu، نويسنده , , Lei and Loor، نويسنده , , Juan J. and Oliveira، نويسنده , , Rosane and Lewin، نويسنده , , Harris A.، نويسنده ,
Pages
11
From page
235
To page
245
Abstract
The strategy used to create and annotate a 7872 cDNA microarray from cattle placenta and spleen cDNA sequences is described. This microarray contains approximately 6300 unique genes, as determined by BLASTN and TBLASTX similarity search against the human and mouse UniGene and draft human genome sequence databases (build 34). Sequences on the array were annotated with gene ontology (GO) terms, thereby facilitating data analysis and interpretation. A total of 3244 genes were annotated with GO terms. The array is rich in sequences encoding transcription factors, signal transducers and cell cycle regulators. Current research being conducted with this array is described, and an overview of planned improvements in our microarray platform for cattle functional genomics is presented.
Keywords
CDNA , Microarray , ANNOTATION , cattle
Journal title
Astroparticle Physics
Record number
2055698
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