Title of article
Vaccination of bovines with recombinant Boophilus Yolk pro-Cathepsin
Author/Authors
Leal، نويسنده , , Alexandre T. and Seixas، نويسنده , , Adriana and Pohl، نويسنده , , Paula C. and Ferreira، نويسنده , , Carlos A.S. and Logullo، نويسنده , , Carlos and Oliveira، نويسنده , , Pedro L. and Farias، نويسنده , , Sandra E. and Termignoni، نويسنده , , Carlos and da Silva Vaz Jr.، نويسنده , , Itabajara and Masuda، نويسنده , , Aoi، نويسنده ,
Pages
5
From page
341
To page
345
Abstract
Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization.
Keywords
Tick vaccine , BYC , aspartic proteinase , cytokine , Bovine , Boophilus microplus
Journal title
Astroparticle Physics
Record number
2056319
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