Title of article :
Assessment of the cell-mediated immunity induced by alphavirus replicon-vectored DNA vaccines against classical swine fever in a mouse model
Author/Authors :
Zhao، نويسنده , , He-Ping and Sun، نويسنده , , Jianfu and Li، نويسنده , , Na and Sun، نويسنده , , Yuan and Xia، نويسنده , , Zhao-He and Wang، نويسنده , , Yu and Cheng، نويسنده , , Dan and Qi، نويسنده , , Qiao-Fen and Jin، نويسنده , , Mei-Lin and Qiu، نويسنده , , Hua-Ji، نويسنده ,
Pages :
9
From page :
57
To page :
65
Abstract :
We have previously shown that an alphavirus replicon-vectored DNA vaccine (pSFV1CS-E2) encoding the E2 glycoprotein of classical swine fever virus (CSFV) completely protected the immunized pigs from lethal challenge. These animals developed only low or moderate level viral-specific antibody titers before challenge, implying that cell-mediated immunity (CMI) probably played an important role in the protective immunity against CSFV conferred by the DNA vaccine. In this study, the CMI induced by pSFV1CS-E2 and its derivative pSFV1CS-E2-UL49 encoding a fusion protein of CSFV E2 and pseudorabies virus (PRV) VP22 was evaluated in a mouse model by lymphoproliferation assays based on CFSE or WST-8, intracellular cytokine staining, and cytokine ELISA. The results showed that both vaccines induced CSFV-specific lymphoproliferative responses and cytokine production, and pSFV1CS-E2-UL49 induced stronger lymphoproliferative responses and higher cytokine levels than pSFV1CS-E2. These findings suggest that the alphavirus replicon-delivered DNA vaccines are capable of inducing CMI, and PRV VP22 is able to enhance the immunogenicity of the co-delivered antigen.
Keywords :
Classical swine fever virus (CSFV) , Cell-mediated immunity (CMI) , VP22 , Alphavirus replicon vector
Journal title :
Astroparticle Physics
Record number :
2059767
Link To Document :
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