Author/Authors :
-، - نويسنده Department of Biochemistry, Pasteur Institute of Iran, Tehran, IR Iran Fayaz, Shima , -، - نويسنده Department of Biochemistry, Pasteur Institute of Iran, Tehran, IR Iran Fard-Esfahani, Pezhman , -، - نويسنده Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran Khaghani, Shahnaz
Abstract :
Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCR-RFLP technique. Materials and Methods: Genomic DNA samples from 350 healthy individuals were obtained. PCR-HRM analysis and PCR-RFLP method were performed simultaneously. Results: Three different melting profiles corresponding to three different genotypes recognized by HRM analysis. The results of PCR-RFLP showed no discrepancy. Conclusions: We concluded that the HRM technique can be used as a screening method for rapid discrimination of R188H genotypes in XRCC2 gene.
