Title of article
Development of a High-Resolution Melting Method for Screening R188H Polymorphism in XRCC2 Gene
Author/Authors
-، - نويسنده Department of Biochemistry, Pasteur Institute of Iran, Tehran, IR Iran Fayaz, Shima , -، - نويسنده Department of Biochemistry, Pasteur Institute of Iran, Tehran, IR Iran Fard-Esfahani, Pezhman , -، - نويسنده Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran Khaghani, Shahnaz
Issue Information
فصلنامه با شماره پیاپی 42 سال 2013
Pages
5
From page
104
To page
108
Abstract
-
Abstract
Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCR-RFLP technique. Materials and Methods: Genomic DNA samples from 350 healthy individuals were obtained. PCR-HRM analysis and PCR-RFLP method were performed simultaneously. Results: Three different melting profiles corresponding to three different genotypes recognized by HRM analysis. The results of PCR-RFLP showed no discrepancy. Conclusions: We concluded that the HRM technique can be used as a screening method for rapid discrimination of R188H genotypes in XRCC2 gene.
Journal title
Iranian Journal of Biotechnology (IJB)
Serial Year
2013
Journal title
Iranian Journal of Biotechnology (IJB)
Record number
2064968
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