Pollutant sensing layer based on cytochrome P450

In this work, cytochrome P4502B4 fusion protein was utilized to realize sensing layers for detection of styrene in atmosphere. Glutathione S-transferase (GST) fusion protein was utilized to realize thin films by Langmuir–Blodgett (LB) horizontal transfer technique. The interaction between the styrene and the sensing layer was monitored by spectrophotometric and by gravimetric measurements. A shift of the Soret peak of cytochrome P450 in the presence of the substrate was found when absorbance measurements were performed. Index of spin state equilibrium variation of P4502B4 was calculated in order to verify the interaction between styrene and cytochrome. A saturation trend in mass density was found when quartzes nanobalance was utilized.