Conductance and electrostriction of bilayer lipid membranes supported on conducting polymer and their application for determination of ammonia and urea

The conductance and electrostriction of bilayer lipid membranes (sBLM) supported on stainless steel and on conducting polymer polypyrrole (PPY) were studied. We showed that an increase in ammonia concentration resulted in an increase in d.c. current flow across the PPY layer adjacent to the metal surface and that covered by the lipid bilayer. The elasticity modulus in the direction perpendicular to the sBLM surface (E) and the electrical capacitance (C) were characterized by a complicated dependence on the application of an external d.c. voltage. In the case of sBLM formed on the PPY layer and that modified by avidin-urease, higher values of E were observed and in addition the latter system was characterized by a less expressed hysteresis of E and C than the metal supported sBLM. This is evidence that sBLM formed on PPY layers is smoother and more homogeneous than that formed on a metal support. We developed a urea minisensor based on sBLM supported on a PPY layer. Avidin-biotin technology was applied for the immobilization of urease on sBLM and the substantial sensitivity of this device for the determination of urea was shown.