Title of article
Imaging of a band for DNA fragment migrating in microchannel on integrated microchip
Author/Authors
Ueda، نويسنده , , Masanori and Nakanishi، نويسنده , , Hiroaki and Tabata، نويسنده , , Osamu and Baba، نويسنده , , Yoshinobu، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2000
Pages
4
From page
33
To page
36
Abstract
We investigated the microfabrication of PCR reaction vessel, capillary array electrophoresis (CAE), and laser-induced fluorescence detection cell using synchrotron radiation in polymethylmethacrylate (PMMA) substrates towards the development of novel technology for ultra-fast DNA analysis. Laser-induced fluorescence detection system for the chip has been developed and successfully applied to the imaging of a band for DNA fragment labeled by fluorescent dye during migrating in the array of microchannels to optimize the separation conditions for DNA analysis. Based on the imaging data, we can estimate that 10-s separation of some DNA fragments will be achievable using 10-mm separation channel on a chip.
Keywords
Migrating , DNA fragment , Imaging
Journal title
Materials Science and Engineering C
Serial Year
2000
Journal title
Materials Science and Engineering C
Record number
2097169
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