Author/Authors :
Murakami، نويسنده , , Yuji and Idegami، نويسنده , , Kotaro and Nagai، نويسنده , , Hidenori and Kikuchi، نويسنده , , Takayuki and Morita، نويسنده , , Yasutaka and Yamamura، نويسنده , , Akira and Yokoyama، نويسنده , , Kenji and Tamiya، نويسنده , , Eiichi، نويسنده ,
Abstract :
Novel methods to construct a multianalyte biosensing chip are described. A method is two-step immobilization in which materials are not immobilized directly but indirectly via small support materials. Biomaterials are immobilized on a certain support particle in the first step. The particles are placed or stuck on a chip in the second step. The other method is random fluidic self-assembly of the microsupports for reducing the complication in the second step on the chip. The combination of the methods enables us to immobilize various kinds of biomaterials densely on a chip. The ways to avoid possible problems due to random distribution are discussed. Several examples are also described.
