Living cells captured on a bio-microsystem devoted to DNA injection

This paper deals with the realization of a microsystem for living cell manipulation. The aim of this research is to make a high-efficiency DNA injection microsystem, thus providing a powerful tool for genetic therapy. crosystem is multifunctional: it is expected to catch ill cells, range them as an array, insert the therapeutic DNA as well as a control gene, detect nontransfected cells for their lysis, and liberate transfected cells for their culture and reinsertion into the body of the patient. For the cell trapping specificity, we use antibodies. For the spatial selectivity of the cell trapping (the cell has to be localized precisely on specified areas of the microsystem), two methods are presented in the paper to pattern the surfaces covered with antibodies. e such a microsystem, we have to integrate two types of technologies: the technology of micromachining to realize the mechanical part of the microsystem (for instance, microcapillaries to lead the gene up to the cell), and the technology that is more related to biochemistry and biology for the cellʹs linking part.