Nanohybrid-layered double hydroxides/urease materials: Synthesis and application to urea biosensors

Nanohybrid [ZnAl]-layered double hydroxides/urease were prepared for the first time using the coprecipitation of enzyme and inorganic matrix. By varying the respective amount of urease and LDH, we obtained hybrid materials with various amount and dispersion rate of active biomolecules. X-ray diffraction and infrared spectroscopy confirm the preservation of the structure of each partner while the morphology properties are in good agreement with the permeability study. These new nanohybrids were applied for the development of urea biosensors. Biosensor responses to urea additions were obtained using capacitance (C vs. V) measurements at urease–LDH biofilm deposited on an insulated semiconductor (IS) structure.