Amperometric biosensor for ethanol detection based on alcohol oxidase immobilised within electrochemically deposited Resydrol film

A new biosensor based on amperometric transducer and alcohol oxidase immobilised in Resydrol polymer for ethanol detection has been developed. The optimal composition and immobilisation conditions for creating active membrane based on alcohol oxidase have been determined. Electrochemical deposition of the polymer film has been achieved by applying the potentiostatic pulse profile consisting of 20 consecutive pulses of + 1900 mV for 0.3 s and − 300 mV for 5 s. The biosensors developed show good analytical characteristics such as reproducibility, operational and storage stability. The minimal detection limit was 3.5 × 10− 2 (% v/v) of ethanol. The biosensor developed has been shown to be a potential for ethanol detection in real alcoholic beverages.