Author/Authors :
Maalouf، نويسنده , , R. and Soldatkin، نويسنده , , Luca A. and Vittori، نويسنده , , O. and Sigaud، نويسنده , , M. and Saikali، نويسنده , , Y. and Chebib، نويسنده , , H. and Loir، نويسنده , , A.S. and Garrélie، نويسنده , , F. and Donnet، نويسنده , , C. and Jaffrezic-Renault، نويسنده , , N.، نويسنده ,
Abstract :
Diamond-like carbon (DLC) electrodes which constitute a new research area in electrochemistry and glassy carbon (GC) electrodes were used as transducers for fabrication of glucose oxidase (GOD) biosensors. The amperometric signal of the enzyme electrode was due to the electro-oxidation of H2O2 generated in the enzyme layer. This work has shown that the detection limit of glucose on GOD/GC electrode is 20 μM while it is 50 μM on GOD/DLC electrode. The sensitivity of GOD/GC electrode decreases 4% after 8 days and we have good repeatability of measurements for GOD/DLC electrode in the same day.
Keywords :
Amperometric biosensor , Glucose oxidase , Diamond-like carbon , glassy carbon