Novel sucrose three-enzyme conductometric biosensor

For the first time a conductometric biosensor for sucrose determination has been developed using a complex three-enzyme (invertase, mutarotase, and glucose oxidase) containing membrane as a sensitive element immobilized on the conductometric interdigitated planar electrodes. The time of measurement of sucrose concentration in the solution was about 1–2 min. The dynamic range of biosensor depends on buffer capacity, being 2 μM–5 mM of sucrose in 5 mM phosphate buffer. The conductometric biosensor developed demonstrates high selectivity, operational stability and reproducibility. The dependence of sensor response on pH and ionic strength of tested solution has been studied in this work. Storage conditions have also been under investigation. The sensor appeared to be eligible towards application in practice.