Author/Authors :
Tonda-Turo، نويسنده , , C. and Cipriani، نويسنده , , E. and Gnavi، نويسنده , , S. and Chiono، نويسنده , , V. and Mattu، نويسنده , , C. and Gentile، نويسنده , , P. and Perroteau، نويسنده , , I. and Zanetti، نويسنده , , M. and Ciardelli، نويسنده , , G.، نويسنده ,
Abstract :
Gelatin (GL) nanofibrous matrices mimicking the complex biological structure of the natural extracellular matrix (ECM) were prepared from aqueous solutions by electrospinning technique. GL nanofibres with a diameter size of around 300 nm were obtained optimising the process and solution parameters. To increase the GL stability in aqueous environment γ-glycidoxypropyltrimethoxysilane (GPTMS) was used as GL crosslinker. GPTMS crosslinking did not modify the nanofibrous matrix morphology: fibre diameter and membrane pores size were 327 ± 45 nm and 1.64 ± 0.37 μm, respectively. The produced GPTMS crosslinked GL nanofibres (GL/GPTMS_NF) were found to support the in vitro adhesion, proliferation and survival of neonatal olfactory bulb ensheating cells (NOBECs).
Keywords :
gelatin , aqueous solution , Nanofibres , Glial cells , electrospinning
