Title of article
A new method for fish leucocyte counting and partial differentiation by flow cytometry
Author/Authors
Inoue، نويسنده , , Takamasa and Moritomo، نويسنده , , Tadaaki and Tamura، نويسنده , , Yumi and Mamiya، نويسنده , , Sachiko and Fujino، نويسنده , , Hiroshi and Nakanishi، نويسنده , , Teruyuki، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2002
Pages
12
From page
379
To page
390
Abstract
A simple and rapid method for analysis of fish blood cells is presented. Carp (Cyprinus carpio) blood was diluted 200 times with Hanksʹ solution containing 1 μg/ml of DiOC6(3) which is a fluorescent, lipophilic dye. After staining for 10 min, the blood cells were measured by a flow cytometer (FACS). Several blood cell populations were identified by different FL-1 (green fluorescence), FSC (forward scatter), and SSC (side scatter) properties. FL-1 v. SSC or FSC v. SSC dot-plot of stained blood cells displayed five separate cell populations: erythrocytes; a mixture of thrombocytes plus lymphocytes; monocytes; neutrophils; and basophils. The number of each type of blood cell counted by the FACS was in good agreement with those counted microscopically.
Keywords
Fish , Carp , blood cell count , fluorescent dyes , flow cytometry
Journal title
Fish and Shellfish Immunology
Serial Year
2002
Journal title
Fish and Shellfish Immunology
Record number
2106733
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