Long-term study of antibody response and injection-site effects of oil adjuvants in Atlantic halibut (Hippoglossus hippoglossus L.)

Atlantic halibut were injected intraperitoneally with human gamma globulin suspended in either phosphate buffered saline, Freunds complete adjuvant or Montanide ISA711 to test the long-term effects of adjuvants. Every month for 12 months up to five animals from each group were sampled. The peritoneal cavity was examined and the adhesion level scored on an arbitrary scale. Serum was also collected and analysed by ELISA for antibodies to human gamma globulin. Results show that whilst FCA produced the highest and fastest antibody response, it also produced the fastest intraperitoneal adhesions, persisting through 11 months. However, the adhesions were not very severe and did not appear to affect the halibut. Crown