PCR detection of Listeria monocytogenes in ‘gravad’ rainbow trout

‘Gravad’ rainbow trout artificially contaminated with Listeria monocytogenes was analyzed by use of a 4 h enrichment period followed by extraction of DNA and PCR amplification. This procedure made it possible to detect 10–100 cfu L. monocytogenes per gram ‘gravad’ rainbow trout, within 12 h. After a prolonged enrichment period of 24 h, numbers as low as 1–10 cfu L. monocytogenes per gram could be detected. The method described may be a useful tool for screening samples of ‘gravad’ rainbow trout for the presence of L. monocytogenes, since it is sensitive, rapid and simple.