Title of article :
The vitronectin gene in rainbow trout: Cloning, expression and phylogenetic analysis
Author/Authors :
Marioli، نويسنده , , Dimitra J. and Zarkadis، نويسنده , , Ioannis K.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
8
From page :
18
To page :
25
Abstract :
Vitronectin is a major cell adhesion glycoprotein that is found in plasma and the extracellular matrix. Vitronectin consists of an N-terminal somatomedin B domain and two hemopexin-like domains and controls functions including cell adhesion, migration, haemostasis and immune defence. In order to study the molecular evolution of the complement lytic pathway regulation, we have cloned and characterized the vitronectin gene from rainbow trout (Oncorhynchus mykiss). The deduced amino acid sequence of trout vitronectin exhibits 45%, 46%, 47% and 63% identity with human, chicken, Xenopus and zebrafish orthologs, respectively. The domain architecture of the trout vitronectin, consisting of a somatomedin B domain and two hemopexin-like domains, resembles that of mammalian vitronectins. Analysis of partial genomic clones shows that trout vitronectin gene exhibits the same exon–intron organization profile as the human ortholog gene. The trout vitronectin gene is probably present as a single copy in the trout genome, showing a differential expression pattern among tissues investigated.
Keywords :
Evolution , comparative immunology , CLONING , Gene organization , mRNA expression , rainbow trout , Vitronectin
Journal title :
Fish and Shellfish Immunology
Serial Year :
2008
Journal title :
Fish and Shellfish Immunology
Record number :
2107906
Link To Document :
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