Title of article :
Molecular cloning and characterization of peroxiredoxin 6 from Chinese mitten crab Eriocheir sinensis
Author/Authors :
Mu، نويسنده , , Changkao and Zhao، نويسنده , , Jianmin and Wang، نويسنده , , Lingling and Song، نويسنده , , Linsheng and Zhang، نويسنده , , Huan and Li، نويسنده , , Chenghua and Qiu، نويسنده , , Limei and Gai، نويسنده , , Yunchao، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Pages :
7
From page :
821
To page :
827
Abstract :
Peroxiredoxin is a superfamily of antioxidative proteins that play important roles in protecting organisms against the toxicity of reactive oxygen species (ROS). In this study, the full-length cDNA encoding peroxiredoxin 6 (designated EsPrx6) was cloned from Chinese mitten crab Eriocheir sinensis by using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of EsPrx6 was of 1076 bp, containing a 5′ untranslated region (UTR) of 69 bp, a 3′ UTR of 347 bp with a poly (A) tail, and an open reading frame (ORF) of 660 bp encoding a polypeptide of 219 amino acids with the predicted molecular weight of 24 kDa. The conserved Prx domain, AhpC domain and the signature of peroxidase catalytic center identified in EsPrx6 strongly suggested that EsPrx6 belonged to the 1-Cys Prx subgroup. Quantitative real-time RT-PCR was employed to assess the mRNA expression of EsPrx6 in various tissues and its temporal expression in haemocytes of crabs challenged with Listonella anguillarum. The mRNA transcript of EsPrx6 could be detected in all the examined tissues with highest expression level in hepatopancreas. The expression level of EsPrx6 in haemocytes was down-regulated after bacterial challenge and significantly decreased compared to the control group at 12 h. As time progressed, the expression level began to increase but did not recover to the original level during the experiment. The results suggested the involvement of EsPrx6 in responses against bacterial infection and further highlighted its functional importance in the immune system of E. sinensis.
Keywords :
Eriocheir sinensis , Peroxiredoxin 6 (Prx6) , innate immunity , mRNA expression , gene cloning , Bacteria challenge
Journal title :
Fish and Shellfish Immunology
Serial Year :
2009
Journal title :
Fish and Shellfish Immunology
Record number :
2108553
Link To Document :
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