Characterization of grass carp (Ctenopharyngodon idellus) Foxp1a/1b/2: Evidence for their involvement in the activation of peripheral blood lymphocyte subpopulations

Foxp subfamily belongs to the Fox family of winged-helix transcription factors and plays critical roles in multiple biological processes including development and immunoregulation. However, little is known about the regulation and function of Foxp subfamily in fish immune system. In this study, we obtained the complete cDNAs of grass carp Foxp1a, Foxp1b and Foxp2. They possess the conserved leucine zipper domain, zinc finger domain and forkhead domain when compared with their mammalian counterparts, except that Foxp1a lacks the forkhead domain. Real-time RT-PCR analysis showed that their transcripts were mainly found in thymus, spleen and peripheral blood lymphocytes (PBLs). In grass carp PBLs, both LPS and PHA were effective in elevating Foxp1b mRNA levels but had no effect on Foxp1a mRNA, while only PHA affected Foxp2 mRNA expression. Using the same cell model, PHA was revealed to up-regulate mRNA expression of T-cell marker genes (CD4-like, CD8α and CD8β) but not B-cell marker gene (IgM). Unlike PHA, LPS increased IgM mRNA level but did not affect T-cell marker gene expression. These findings suggest that PHA and LPS may act on distinct lymphocyte subpopulations in grass carp PBLs and provide evidence for the involvement of Foxp1b and Foxp2 in the activation of different lymphocyte subpopulations in grass carp.