Title of article :
Effects of berberine on arylamine N-acetyltransferase activity in human bladder tumour cells
Author/Authors :
Chung، نويسنده , , J.G. and Wu، نويسنده , , L.T and Chu، نويسنده , , C.B and Jan، نويسنده , , Hueisch-Jy and Ho، نويسنده , , C.C and Tsou، نويسنده , , Vivian M.F. and Lu، نويسنده , , H.F and Chen، نويسنده , , G.W. and Lin، نويسنده , , J.G. and Wang، نويسنده , , T.F، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1999
Abstract :
Berberine was used to determine inhibition of arylamine N-acetyltransferase (NAT) activity in human bladder tumour cells. The NAT activity was measured by HPLC assaying for the amounts of N-acetyl-2-aminofluorene (AAF) and N-acetyl-p-aminobenzoic acid (N-Ac-PABA) and remaining 2-aminofluorene (AF) and p-aminobenzoic acid (PABA). Two assay systems were performed, one with cellular cytosols, the other with intact bladder tumour cell suspensions. The NAT activity in human bladder tumour cells was inhibited by berberine in a dose-dependent manner, that is, the higher the concentration of berberine, the higher the inhibition of NAT activity. The values of apparent Km and Vmax calculated from cytosol NAT and intact cells were also decreased by berberine. This report is the first demonstration to show berberine did affect human bladder tumour cell NAT activity.
Keywords :
2-aminofluorene , N-Acetyltransferase , N-acetyl-2-aminofluorene , N-Acetyl-p-aminobenzoic acid , p-aminobenzoic , Berberine , human bladder cell
Journal title :
Food and Chemical Toxicology
Journal title :
Food and Chemical Toxicology