Title of article :
Investigations on cell proliferation in B6C3F1 mouse liver by diethanolamine
Author/Authors :
Mellert، نويسنده , , W. E. Kaufmann، نويسنده , , W. and Rossbacher، نويسنده , , R. and van Ravenzwaay، نويسنده , , B.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
8
From page :
127
To page :
134
Abstract :
Diethanolamine (DEA) has been shown to induce liver tumours in B6C3F1 mice in a previous 2-year dermal study. To elucidate the mode of action groups of eight male and eight female B6C3F1 mice were dermally exposed to daily DEA doses of 0 or 160 mg/kg body weight/day for 1 week. Reversibility was assessed after a 3-week treatment-free recovery period. Subsequently groups of 10 male B6C3F1 mice were dermally exposed to daily DEA doses of 0 or 160 mg/kg body weight for 1, 4 or 13 weeks. Finally, groups of 8 male B6C3F1 mice were dermally exposed to daily DEA doses of 0, 10, 20, 40, 80, and 160 mg/kg body weight for 1 and 13 weeks. Following a 1-week treatment, DEA caused increased cell proliferation (5-bromo-2′-deoxyuridine (BrdU) method) in zone 3 (central vein region) of the liver lobules at 160 mg/kg body weight. Reversibility of liver cell proliferation was demonstrated in the recovery phase. In the subsequent studies increased cell proliferation was observed at 10 mg/kg body weight or higher after 13 weeks of treatment. These results support the hypothesis that sustained liver cell proliferation is a potential non genotoxic mode of action by which DEA promotes liver tumours in B6C3F1 mice.
Keywords :
Tumour , Cell Proliferation , apoptosis , diethanolamine , mouse , Liver
Journal title :
Food and Chemical Toxicology
Serial Year :
2004
Journal title :
Food and Chemical Toxicology
Record number :
2117746
Link To Document :
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