Author/Authors :
Delibato، نويسنده , , Elisabetta and Rodriguez-Lazaro، نويسنده , , David and Gianfranceschi، نويسنده , , Monica and De Cesare، نويسنده , , Alessandra and Comin، نويسنده , , Damiano and Gattuso، نويسنده , , Antonietta and Hernandez، نويسنده , , Marta and Sonnessa، نويسنده , , Michele and Pasquali، نويسنده , , Frédérique and Sreter-Lancz، نويسنده , , Zuzsanna and Saiz-Abajo، نويسنده , , Mar?a-José and Pérez-De-Juan، نويسنده , , Javier and Butr?n، نويسنده , , Javier and Prukner-Radovcic، نويسنده , , Estella and Horvatek Tomic، نويسنده , , Danijela and Johannessen، نويسنده , , Gro S. and Jako?i?n?، نويسنده , , D?iuginta and Olsen، نويسنده , , John E. and Chemaly، نويسنده , , Marianne and Le Gall، نويسنده , , Francoise and Gonz?lez-Garc?a، نويسنده , , Patricia and Lettini، نويسنده , , Antonia Anna and Lukac، نويسنده , , Maja and Quesne، نويسنده , , Segolénè and Zampieron، نويسنده , , Claudia and De Santis، نويسنده , , Paola and Lovari، نويسنده , , Sarah and Bertasi، نويسنده , , Barbara and Pavoni، نويسنده , , Enrico and Proroga، نويسنده , , Yolande T.R. and Capuano، نويسنده , , Federico and Manfreda، نويسنده , , Gerardo and De Medici، نويسنده , , Dario، نويسنده ,
Abstract :
The classical microbiological method for detection of Salmonella spp. requires more than five days for final confirmation, and consequently there is a need for an alternative methodology for detection of this pathogen particularly in those food categories with a short shelf-life. This study presents an international (at European level) ISO 16140-based validation study of a non-proprietary Real-Time PCR-based method that can generate final results the day following sample analysis. It is based on an ISO compatible enrichment coupled to an easy and inexpensive DNA extraction and a consolidated Real-Time PCR assay. Thirteen laboratories from seven European Countries participated to this trial, and pork meat was selected as food model. The limit of detection observed was down to 10 CFU per 25 g of sample, showing excellent concordance and accordance values between samples and laboratories (100%). In addition, excellent values were obtained for relative accuracy, specificity and sensitivity (100%) when the results obtained for the Real-Time PCR-based methods were compared to those of the ISO 6579:2002 standard method. The results of this international trial demonstrate that the evaluated Real-Time PCR-based method represents an excellent alternative to the ISO standard. In fact, it shows an equal and solid performance as well as it reduces dramatically the extent of the analytical process, and can be easily implemented routinely by the Competent Authorities and Food Industry laboratories.
