Further data on the presence of Fusarium emerging mycotoxins enniatins, fusaproliferin and beauvericin in cereals available on the Spanish markets

In this work, 64 samples of cereals purchased from local markets in the Valencian community (Spain) were investigated for the presence of six emerging mycotoxins: enniatins ENs (ENA, ENA1, ENB and ENB1), beauvericin (BEA) and fusaproliferin (FUS). Samples were extracted with a mixture of water/acetonitrile (85/15, v/v) by using an Ultra-turrax homogenizer. Mycotoxins were then identified and quantified with a liquid chromatography (LC) with diode array detector (DAD). Positive samples were confirmed with an LC–MS/MS. Analytical Results showed that the frequencies of contamination of samples with ENs, BEA and FUS were 73.4%, 32.8% and 7.8%, respectively. ENA1 was the most mycotoxin found and levels ranged from 33.38 to 814.42 mg/kg. ENB levels ranged between 2.23 and 21.37 mg/kg. ENB1 levels varied from 4.34 to 45.94 mg/kg. All samples were free of ENA. BEA levels ranged from 0.51 to 11.78 mg/kg and FUS levels varied between 1.01 and 6.63 mg/kg. It could be concluded from this study that the high contamination levels found especially for ENs could be of a negative impact on the population. This is the first paper on the presence of emerging mycotoxins in cereals available in Spain.