Secondary plant substances in various extracts of the leaves, fruits, stem and bark of Caraipa densifolia Mart.

Thirty secondary plant substances were detected in various extracts of the leaves, fruits, stem and bark of Caraipa densifolia Mart. Phenolic compounds were preliminarily identified and quantitated by HPLC–ESI-MS and the structures of the compounds, purified by semi-preparative HPLC, were further characterized by nano-ESI-MS–MS. The presence of gallic acid, 3,4-dihydroxybenzoic acid, neochlorogenic acid, chlorogenic acid, methyl gallate, p-coumaric acid quinate, epicatechin, procaynidin dimer B2, procyanidin trimer C1, syringic acid, 1,2,3,6-tetragallate glucoside, 1,3,4,6-tetragallate glucoside, corilagin, ellagic acid, methyl ellagic acid rhamnoside, quercetin-3-O-rhamnoside, two apigenin-C-glycosides (vitexin and isovitexin) and two luteolin-C-glycosides (orientin and isoorientin) are reported in this species for the first time. In addition, the previously reported following terpenoids, lupeol, lupenone, betulinic acid, betulin, friedelin and a previously non-characterized terpenoid in this species, friedelinol were identified and quantitated by GC–MS. A previously identified sterol was β-sitosterol along with stigmasterol in this species for the first time. The vitamins α-tocopherol and γ-tocopherol were also identified in extracts of the leaves of Caraipa species for the first time. The data shows that the botanical parts of C. densifolia Mart. has a much richer spectrum of secondary plant substances than previously reported.