Title of article :
Overexpression of Metastatic Related MicroRNAs, Mir-335 and Mir- 10b, by Staphylococcal Enterotoxin B in the Metastatic Breast Cancer Cell Line
Author/Authors :
Foad Heidary، Mohammad نويسنده Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. , , Mahmoodzadeh Hosseini، Hamideh نويسنده Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran , , Mehdizadeh Aghdam، Elnaz نويسنده Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. , , Nourani، Mohammad Reza نويسنده Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran , , Ranjbar، Reza نويسنده Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. , , Mirnejad، Reza نويسنده Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Mirnejad, Reza , Imani Fooladi، Abbas Ali نويسنده ,
Abstract :
Purpose: One of the advanced cancer therapy strategies is immune-stimulating compound
based immunotherapy Staphylococcal enterotoxin B (SEB) is one of the potent
superantigens, which can efficiently activate antitumor immune response to eradicate tumor
growth and inhibit metastasis. Herein, we evaluated the effect of SEB on the expression of
two master microRNAs, mir-335 and mir-10b, involved in metastasis.
Methods: A metastatic breast cancer cell line MDA-MB231was treated with four different
concentrations of SEB, including 10, 102, 103 and 104 ng/ml, for 24 and 48 hours. To
identify the cytotoxic effect of SEB, treated cells were examined by MTT assay. The stem
loop RT-PCR (TaqMan) was used to analyze the mir-335 and mir-10b expression.
Results: Results showed that SEB significantly increased the expression of mir-335 both
after 24 and 48 hours (pv < 0.001 and pv < 0.05, respectively). No significant differences
were found in the mir-10b expression.
Conclusion: Moreover, our findings demonstrated no cytotoxic effect of SEB on the treated
cells. Our results suggest that SEB probably induces its anti-metastatic effect via the
expression regulation of the main genes which contributes to metastasis.
